| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Evans, Olivia M (10)
|
MASEA |
31.60 |
32.80 |
1.20 |
| 2 |
Brandt, Mackenzie A (10)
|
MAWAC |
34.51 |
32.97 |
-1.54 |
| 3 |
Waslyn, Rylee E (10)
|
MAWAC |
33.38 |
33.38 |
0.00 |
| 4 |
Sandock, Leah J (10)
|
MALAC |
33.62 |
33.69 |
0.07 |
| 5 |
Feuzeu Mekoue, Laurielle Kel (10)
|
MASSC |
34.48 |
34.33 |
-0.15 |
| 6 |
Eruslanova, Ekaterina (10)
|
MASSC |
35.66 |
34.36 |
-1.30 |
| 7 |
McNulty, Mary Frances T (10)
|
MACBST |
35.27 |
34.72 |
-0.55 |
| 8 |
Zakarevicz, Kaily M (10)
|
MAUN-2 |
35.04 |
34.89 |
-0.15 |
| 9 |
Seiverd, Ally E (10)
|
MASEA |
37.07 |
34.94 |
-2.13 |
| 10 |
Hahn, Lily (9)
|
MARAD |
36.68 |
35.23 |
-1.45 |
| 11 |
Saul, Yuran A (10)
|
MAJRAY |
35.38 |
35.83 |
0.45 |
| 12 |
Bockrath, Rachel K (9)
|
MAWAC |
36.68 |
36.59 |
-0.09 |
| 13 |
Ramsey, Lauren M (9)
|
MAAAC |
36.45 |
37.64 |
1.19 |
| 14 |
Shifflett, Ella M (10)
|
MASSC |
36.36 |
37.73 |
1.37 |
|
Shaffer, Marissa K (10)
|
MAPCAC |
36.19 |
NS |
- |